|
KMID : 1147820120010010077
|
|
Journal of Naturopathy
2012 Volume.1 No. 1 p.77 ~ p.83
|
|
|
Biocide Effects of Wood Vinegars against Skin Microorganisms
|
|
Kim Yoo-Bok
Na Sun-Hee
Kim Soo-Young
Lee Hyung-Hwan
|
|
|
Abstract
|
|
|
|
The purpose of this study is to examine the growth inhibition of fungi or bacteria and the sterilizing capacity of wood vinegar a natural material to use it for the natural therapy. The wood vinegar is made from the liquid obtained by natural cooling of smoke coming out when the wood becomes the charcoal or by dry distillation of wood chips. For this study, 6 types of wood vinegar (sample-2, 2.2, 3, 4, 5 and 5-1) were prepared. Above wood vinegars had some effect to restrict the multiplication of athlete's foot fungi T. mentagrophytes & T. rubrum in the anti-fungus disk diffusion method, and the effect of sample-5(mechanical wood vinegar) was the best. In the anti-biotic disk diffusion method of above wood vinegars, all the wood vinegars showed the effect to restrict the multiplication of bacteria such as S. aureus, E. coli, A. baumannii, B. epidermidis and C. xerosis. Especially, sample-5 with the growth inhibition zone of 18-35mm size was the strongest anti-biotic. In the liquid medium of above wood vinegars, the minimum inhibitory concentration(MIC) of sample-2, 2.2, 3 and 4 against the athlete's foot fungus T. rubrum was 6.25%, and that of sample-5(mechanical type) was 1.5%. Thus, the inhibitory capacity of sample-5 was the best, too. The MIC of bacterial growth inhibition was 1.56% for S. aureus, E. coli, A. baumannii and B. epidermidis and 0.78% for C. xerosis, in which the effect of sample-5 was the best. The minimum bactericidal concentration(MBC) of sample-5 was 0.78% for S. aureus, C. xerosis and A. baumannii, and 1.56% for E. coli and B. epidermidis. Thus, its bactericidal power was the best among 6 wood vinegars. Wood vinegars sample-2 and 5 were solved in water, chloroform, hexane, butanol and ethyl acetate respectively and divided into separate fractions to treat above-mentioned 5 bacteria with them. As the result, all the samples showed the lethality. S. aureus showed the highest lethality in the chloroform fraction of sample-2 with 0.156 mg/ml of MIC, and its average lethality was high in order of ethyl acetate, chloroform, hexane. Sample-2 and 5 were mixed into chloroform and ethyl acetate to get 11 fractions. In MIC, fraction-4 had the highest lethality; its MBC was 0.156 mg/ml for S. aureus, 0.625 mg/ml for E. coli1, 0.156 mg/ml for A. baumannii, 0.156 mg/ml for B. epidermidis, and 0.078 mg/ml for C. xerosis. With the silica gel column, 11 fractions were obtained from sample-2. Among these fractions, the lines-2, 3, 4 and 5 of fraction-5 with high anti-biotic capacity were analyzed in Sephadex column chromatography. Then, after the MPLC treatment, they were analyzed with the ion spectra to find the single peak of 127 molecular weight. In conclusion, the wood vinegars may be a useful material for natural healing and microbial suppression on human body.
|
|
|
KEYWORD
|
|
|
chloroform, chromatography, wood vinegar, mycosis, bacteria, disc diffusion, minimum inhibition concentration, chloroform, chromatography
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|